getSpliced() error: improper genomic coordinate 46954453 on chrUn_random for TCONS_00084296

@tikshana_yadav

This problem looks like a genome mismatch issue. Data was mapped against one version of the genome but the annotation was based on a different one? My guess is both happened to have a “chrUn_random” chromosome but they are different lengths, which means different content. All of the coordinates in your data will be wrong if this is true – and the tool will not necessarily error, just produce scientifically incorrect results.

That said, all of these tools are deprecated when used in Galaxy, as explained in your other post here:

Why and the alternatives are covered in this prior Q&A:

If you want to try to troubleshoot line-command usage, there was a google group – I don’t think it is very active anymore but you may find prior help. Biostars, Seqanswers, and other general bioinformatics forums will also have much prior discussion. These are usually common enough that even just a basic web search with the error message quoted will find help.

But avoid using the tools in Galaxy, or expected problems.

Thanks!