Hi @ding66
Compare this:
- List of genes with the true/false
- Your “The GO annotation file that I uploaded to goseq” file
- Do both use the same gene ID format?
If there is a mismatch, that is likely the problem. You would need to adjust the file in step 2 above to match your existing list of genes from step 1. A “replace” tool could do that if you can find the mapping. Data Manipulation Olympics
I might be guessing wrong, but you can share all the inputs and we can look at this closer.