Getting Data into Galaxy

Data Upload

Use the Upload tool to load datasets into your history

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  • Files from your computer using Choose local files
  • Files with a public URL using Paste/Fetch data
  • Files from remote data sources using Choose remote files

:flashlight: files can be dragged and dropped to launch the Upload tool

More choices include

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  • Use a data retrieval tool to load files into your history. See the tool panel for these options
  • Copy datasets from Data → Data Libraries into your history

Batch Upload

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All servers support a resume function directly through the Upload tool for very large local data loading.

Data retrieved through all other methods is entirely remote and performance is dependent on the connection between that other service and the Galaxy server where you are working.

UseGalaxy.org offers the custom command-line galaxy-upload function.

FTP Upload is available at some services, including UseGalaxy.eu and UseGalaxy.org.au.


Q&A

:speech_balloon: I tried to retrieve a batch of accessions from NCBI SRA, but the query failed!

First, check your query terms. Most of these underlying tools expect one accession term per line without any extra spaces or blank lines. You can confirm by reviewing the tool form instructions.

Next, try again! External data services can get busy and reject queries for unclear reasons. Trying again at a different time is a common solution.


:books: References


:mechanic: FAQ: What information should I include when reporting a problem?

Any persistent problems can be reported in a new question for community help. Be sure to provide enough context so others can review the situation exactly and quickly offer advice.

Consider FAQ: Sharing your History or posting content from the Job Information :information_source: view as described in FAQ: Troubleshooting errors.

RNA-Seq analysis in Galaxy
How to downsize my 4Go FASTQ file to an 1Go FASTQ file?
DiffBind - run not completing
Download and Extract Reads in FASTQ: Troubleshooting Fatal error: Exit code 2 ()
Problem with files waiting to be downloaded
Fastq files not uploading
Uploading to usegalaxy.org
FAQ: NCBI reference data
Help me with an error with uploading my dataset
Allocation of server for teaching a class since the speed is low
AquaINFRA OGC API Processes
Length of Quality Sequence and Length of read do not match
Kraken2 Not supporting the required formats for paired sequencing
Help with interpreting GO enrichment resutls using goseq
Job failure with “sqlite3.OperationalError: unable to open database file” on usegalaxy.org
Trouble Uploading Large Files
upload error from Globus
At uploading got "Error Content Not Available"
Large discrepancies between processing identical files on Org vs EU site
Minimap2 accepted formats
Repeatexplorer fails on single end input
Uploading files
Tsv file upload fail for maaslin2
Bismark mapping with Fatal Error exit
Upload files stuck in queue
ENA submission receipt: Excel input formatting and troubleshooting
Error in using FastQC in Galaxy EU
Upload data directly into a collection! Solves: No compatible list of pairs dataset collections available
NCBI BLAST+ blastn
GOEnrichment analysis
Fastqcdump file issue
data not uploading
GTF2GeneList finds features but stores 0 bytes in annotation.txt
Error in running braker3
Uploading large file problem
Getting errors for Salmon
Usage help -- single end versus paired end BAM options
Convert GTF to BED12
Help with interpreting GO enrichment resutls using goseq
Error of Deseq2