Hello @hashimoto.takuma
This tool and most other Bioconductor tools require replicates.
FAQ with related tips: Extended Help for Differential Expression Analysis Tools
Give that a review and maybe reorganize your sample data?
If you run into more problems, you can post back the job details and we can try to help more. The part with the inputs and parameters is the most important (“i” icon). Try to leave everything intact with a full copy/paste or screenshot.