I’m currently in the process of performing DEGS idenitification with DESEQ2 in the galaxy pipeline.
I performed all my steps with Galaxy.
I performed whole RNA transcriptome sequencing. The obtained FASTq files I imported into galaxy and then I performed the following steps:
And I try DESeq2. But few minits later,I got error meassage.I checked “Tool Standard Error” and it says :
estimating size factors
Error in checkForExperimentalReplicates(object, modelMatrix) :
The design matrix has the same number of samples and coefficients to fit,
so estimation of dispersion is not possible. Treating samples
as replicates was deprecated in v1.20 and no longer supported since v1.22.
Calls: DESeq … estimateDispersions → .local → checkForExperimentalReplicates
Please give some advices,help me.