I obtained the transcript count file using StringTie and now I am trying to run the count file on DeSeq2 using galaxy server. I am getting the following error.
The design matrix has the same number of samples and coefficients to fit, so estimation of dispersion is not possible. Treating samples
as replicates was deprecated in v1.20 and no longer supported since v1.22.
I don’t know how to fix this error, please help!