I’ve tried uploading some fastqsanger files onto HISAT2. I have done this operation many times previously with the same data and it was able to work effectively. However, this time the operation has failed and this warning note came up:
Remote job server indicated a problem running or monitoring this job.
You’ve tried re-uploading obviously haven’t you? I have known mysterious corruption occurring to a fastsanger between downloading to my workstation and uploading to my Galaxy server (ie first clue was strange HiSAT2 behaviour). Never pinned down where that happened, but redoing the whole process fixed it?