How to normalised ChIP-Seq data set with Spike-in

I am using ChIP-dataset with drosophila spike-in. But can not find out which tool I should use for the spike in normalization in Galaxy.
Can you kindly help me.
With regards

Hello @moonmoondeb

Several of these tutorials include discussion about how to process and interpret normal versus treated samples → Epigenetics / Tutorial List

Other than that, maybe find a few publications that process the data they way you also want to, then use the same or similar processes in Galaxy? If you can share the tool, process, and publication that will make it easier for people at this forum to suggest analogous methods. :slight_smile: