Hi everyone,
I have a BAM file in the following format (some of the data).
I would like to subset my BAM via specific cell barcode sequences (e.g, AAAGAACCAGGACATG, AACCAACAGAGATTCA, GCCATTCTCACAGTGT) into a new BAM.
Is there any way to do so efficiently?
It would be great if someone knew how to do this.
Stay healthy. Many thanks,
Miae
Hi Miae
If you are just interested in a particular cell barcode, have you tried “Select lines that match an expression” (https://usegalaxy.eu/root?tool_id=Grep1), assuming your file is actually in SAM format.
Regards, Hans-Rudolf
Hi Hans-Rudolf,
It worked (:D) I am a beginner who has just started using Galaxy. I am getting a lot of help through Galaxy Help. Thank you very much.