hello, I have loaded my sequences in galaxy because I want to do 16S Microbial Analysis with Mothur, and in the step of make.contings, the files are not created, a message that says “This job is currently running” appears, many hours have passed but Many, I do not know if I’m doing something wrong or it’s normal, could you help me please?
Which Galaxy server are you using? In general bioinformatic tools can run a long long time, even days. This is dependent on the tool in question, the size of the input data size and how busy the server is. So I wouldn’t worry and wait a little bit longer.
You also might want to have a look at our training material about 16S analysis with Mothur.