I’m having trouble when using MITObim. I am uploading my FASTA reference sequence and my read pool in fastq format (R1 and R2 concatenated in one file). I always get the same error:
An error occurred with this dataset:
format fasta, database ?
I guess the problem should be with my fasta sequence, but it seems to be ok. Does anyone know how to proceed with MITObim
Welcome, @idoia_jim
You could try running a tool like Fasta Statistics on your uploaded fasta file to see what that reports. Link to the tool at the EU server → fasta_stats
Sometimes a tool will want the sequence identifiers (only) on the > title lines. There can be other minor issues with line wrapping, too. This guide includes some things to check/adjust and the tools involved. → Datatypes - Galaxy Community Hub
If that is not enough and you would like more help, please share back some more details about the job. What we will be looking for is in the banner at this forum, also here. → How to get faster help with your question
Let’s start there, and let us know if you solve this! 