NGS analysis for mRNA display

Hi, I am very new to NGS analysis, and I am afraid I am not good with programming, which is why people recommended Galaxy. I have only two questions so far:

  1. I got my Illumina sequencing results, and the reads are scrambled from 5’ to 3’ and 3’ to 5’. Is there a way to sort them in the same orientation?

  2. I already did FASTA QC, and it looks like my results are decent; I want to do some editing, such as trimming and counting sequence populations. Can I do this on Galaxy? If yes, could the community help me with the flowthrough, or are there tutorials for this specific reason?

Any help is welcome; thanks for your time.

Hi @Estheban050
start with
Kind regards,