I have the raw illumina paired-end .fastq reads from a whole genome sequencing of my own genome done by a sequencing company. All the raw reads are about 50Gb in size. Would I be able to use the https://usegalaxy.org/ free site to do the FastQC assessment, adapter/read trimming, alignment to the reference genome and some basic form of variant calling using just the free site alone? I have a biology background and have some experience using computational biology tools, but I have never tried to assemble my own genome and I hope this could be a good learning experience for me. Does Galaxy have a tutorial for doing an assembly to hg19 reference genome using my own whole genome sequencing data?
Thank you so much for the help.