normalization of counts with only 1 condition - DESeq2 can be used? otherwise, how I can do this?

1

Hi,
I have a question about the data normalization in DESeq2.
I have a matrix of gene counts from 30 samples belonging to the same group. I don’t want to get DE genes obviously. I just need to normalize these counts. Is there a way to do this in DESeq2 without the need to specify two conditions?

Thanks
Barbara

2

The tool needs at least two conditions each with replicates or there is nothing to compare for generated statistical tests.

Details direct from the original tool authors are in this post and related posts at their forum: How to perform DEseq2 on datasets without replicates or merged replicates