Hi, I am confused why I am getting this error:
Traceback (most recent call last):
File "/usr/local/tools/_conda/envs/mulled-v1-df158f7db8ef004e884a3706d733d0a108cdd0c74ff386f5830ed8d37c0f63ec/bin/peakachu", line 161, in <module>
sys.exit(main())
File "/usr/local/tools/_conda/envs/mulled-v1-df158f7db8ef004e884a3706d733d0a108cdd0c74ff386f5830ed8d37c0f63ec/bin/peakachu", line 140, in main
args.func(controller)
File "/usr/local/tools/_conda/envs/mulled-v1-df158f7db8ef004e884a3706d733d0a108cdd0c74ff386f5830ed8d37c0f63ec/bin/peakachu", line 150, in adaptive_approach
controller.adaptive_approach()
File "/usr/local/tools/_conda/envs/mulled-v1-df158f7db8ef004e884a3706d733d0a108cdd0c74ff386f5830ed8d37c0f63ec/lib/python3.6/site-packages/peakachulib/controller.py", line 160, in adaptive_approach
self._calc_sig_peaks_adaptive(adaptive, size_factors)
File "/usr/local/tools/_conda/envs/mulled-v1-df158f7db8ef004e884a3706d733d0a108cdd0c74ff386f5830ed8d37c0f63ec/lib/python3.6/site-packages/peakachulib/controller.py", line 247, in _calc_sig_peaks_adaptive
self._args.pairwise_replicates)
File "/usr/local/tools/_conda/envs/mulled-v1-df158f7db8ef004e884a3706d733d0a108cdd0c74ff386f5830ed8d37c0f63ec/lib/python3.6/site-packages/peakachulib/adaptive.py", line 285, in run_deseq2_analysis
pairwise_replicates)
File "/usr/local/tools/_conda/envs/mulled-v1-df158f7db8ef004e884a3706d733d0a108cdd0c74ff386f5830ed8d37c0f63ec/lib/python3.6/site-packages/peakachulib/deseq2.py", line 35, in run_deseq2
dds = r.estimateSizeFactors(dds)
File "/usr/local/tools/_conda/envs/mulled-v1-df158f7db8ef004e884a3706d733d0a108cdd0c74ff386f5830ed8d37c0f63ec/lib/python3.6/site-packages/rpy2/robjects/functions.py", line 178, in __call__
return super(SignatureTranslatedFunction, self).__call__(*args, **kwargs)
File "/usr/local/tools/_conda/envs/mulled-v1-df158f7db8ef004e884a3706d733d0a108cdd0c74ff386f5830ed8d37c0f63ec/lib/python3.6/site-packages/rpy2/robjects/functions.py", line 106, in __call__
res = super(Function, self).__call__(*new_args, **new_kwargs)
rpy2.rinterface.RRuntimeError: Error in estimateSizeFactorsForMatrix(counts(object), locfunc = locfunc, :
every gene contains at least one zero, cannot compute log geometric means