I have worked through the Single-Batch Processing hand-on tutorial three times and consistently get different results than what the tutorial says. After the UMI-tool count (last step of the Single-Batch Processing), the tutorial states there should be 2,140 lines in the matrix and 180 columns, but I am consistently getting 1,972 lines and 50 columns.
Is anyone else experiencing this?
Does anyone have any ideas why this is happening? I made sure to use the Tutorial Mode on galaxy, so when I would select the next step it took me to the appropriate tool and version.
I contacted the people who developed the training; probably they will provide you an answer in a few days.
Hi @swren, thanks for trying out the training, could you share your history with me so that I might be able to debug it? My user-name is “mehmet-tekman”
I will be out of office for a few days, but will happily look at your history when I am back.
Hi @mtekman, thank you for reaching out! Here is the link to my history: Galaxy | Europe | Accessible History | Preprocessing of scRNA Data
I have two tries on this history, and the second try is the one that I used tutorial mode for the links to the tools.
Dear @swren - I am back from holiday, thanks for sharing your history.
Can you try the analysis again, this time using slightly different input FASTQ files:
(note the lack of “_subset” prefix)