When I try Lefse analysis, I often get this kind of error “Job ran longer than the maximum allowed execution time (runtime: 0:15:15, limit: 00:15:00), please try different inputs or parameters”.
I think you should look at the number of rows rather than the file size.
By the way, I just checked one of my inputs, which is 269-lines long and 70kb, far smaller than yours.
Actuality, the number of columns will influence too; mine has 17 columns.
I mean, how many columns/rows is your input?
Whoa…Big boy of a file you got there. I’ve never tried anything close to this.
Can you partition this based on your variables? Or even filter it based on abundance/etc before going to LEfSe?