Hi @liuyushu
The tool you are using is specific to the http://huttenhower.sph.harvard.edu/galaxy/
domain-specific public Galaxy server.
The tools and protocols hosted there differ significantly from the Lefse
tools hosted at most other public Galaxy servers (including usegalaxy.* servers).
The administrators of that particular public Galaxy server do not track this forum. Instead, contact them directly, after double-checking your protocol and inputs against the help/tutorials they offer (below).
- Contact: Huttenhower Lab
Help/tutorials: (also linked from the server’s home page, with example data available):
Prior Q&A and other related resources including other versions of this tool and different tools that may be helpful when working with this type of data:
- Lefse Cladogram prior Q&A at this forum: Search results for 'Cladogram' - Galaxy Community Help
- All Galaxy resources: Searching the Galaxy
I also added a tag for that public server to your post. Click on the tag to review all Q&A at this forum related to troubleshooting and help/content for that server.