Hi,
I’m looking for a tool/way to extract the barcode from fastq file and to split fastq file into separate fastq files based on the indices. The indices are placed in the sequence ID. Here is the example:
@NB552014:62:HGCLWBGXJ:1:11101:8614:1055 1:N:0:ACTATAAC+TAAGATTA
GATCGNAAGAGCACACGTCTGAACTCCAGTCACACTA
+
AAAAA#EEEAEEEEEEAEE/AEEEEEAE//EE</A//
Incorrect index sequences were entered in the sample sheet and Illumina demultiplexing filed. I have the fastq files and try to demultiplex on my own…any suggestions, please?