I used the infer experiment on Galaxy platform to know the strandedness of my single end read RNA sequencing dataset.
It gave me the following output:
This is SingleEnd Data
Fraction of reads failed to determine: 0.0286
Fraction of reads explained by “++,–”: 0.0102
Fraction of reads explained by “±,-+”: 0.9612
What can I know about the strandedness of this library? Is it forward or reverse reads?