What tool can I use in Galaxy to check for variance between my replicates to know if I need to exclude anyone from my analysis?
Hi @NIKITA_JHAVERI,
you can calculate the variance of each replicate (e.g. for each featureCounts output) by using the Datamash tool. As an alternative approach, you can visualize the variability of each replicate by using a boxplot graph. Although it doesn’t provide information about variances, it provides other measures of spread, such as the IQR and range. In this history is detailed how to generate it.
Regards.
Hello,
Thank you for your reply. I am not able to access the history you have shared with me. Can you please share it again?
Hi @NIKITA_JHAVERI,
here is Galaxy | Europe | Accessible History | Boxplot RNA-seq. Let me know if you can access now.
Regards
I can. Thank you