Hi @katiem_162
Would you please share more details about your server and configuration?
What to start with
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The release of Galaxy you are running. Using a stable release is recommended. → Releases — Galaxy Project 24.0.3.dev0 documentation
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Are all of these tools recent installations, or did some work “before” and are now not working? Meaning, have jobs ever worked?
- I think you are saying that Trinity runs, but BAMTools doesn’t – is that new behavior? (as a guess, that could indicate a problem with Samtools or data indexes). What are you error messages? Find these using the i-icon within an error dataset.
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Have you made any changes to Galaxy or the server environment between the functioning and non-functioning? Can you share what that was?
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Are you installing from the Galaxy ToolShed? Did you use the managed dependencies option (strongly recommended!)? Are your server dependencies at the default or have you customized these (and how?)?
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Note: many any will find the updated Galaxy Docker release as a less complicated alternative. This is now at a current Galaxy release and the README has been updated – so don’t miss that!
Let’s start there, thanks!
References