Hi @jennaj , thanks heaps. Yes anonymity has been a problem I did not realize at first. I managed to import FASTQ files and imported/undertook analysis via QIIME2 tools for few samples (only one by one :)). Uploading via rule-based uploader is appearing to be a challenge as my data has no URL associated to it (uploading locally). I saw in one of your reply/comment to a question that if a file path can be used once tsv file is created to in this thread (Using local files in rule based uploader). I followed those steps: created tsv file with sequence and file path columns. In building rules for uploading condition, I put column A as list identifier, however, “file path” or to that effect is not available for column B (source identifier). Available options are URL, type, etc. I tried in all options…well…expectedly did not work. I know I am all over the place…but if there is any tip, I immensely appreciate it 