I am currently using the Galaxy Europe platform to analyze 16S sequencing data, while using the 16S Microbial Analysis tutorial (https://galaxyproject.github.io/training-material/topics/metagenomics/tutorials/mothur-miseq-sop/tutorial.html#otu-clustering). I have reached the step where the data is normalized using the sub.sample tool. I have followed the procedure provided in the tutorial and used the following parameters within the sub.sample tool:
- “Select type of data to subsample”: OTU Shared
- “Shared”: The “shared” file from Make.shared tool
- “Size”: 3242
However, after running the tool several times I receive an empty list with an error indicator in red rather. As a result, I am unable to proceed with the analysis.
I have also received an error message from the Galaxy platform mentioning the following:
“An error occurred while updating information with the server. Please contact a Galaxy administrator if the problem persists.”
Is it possible that this error message is linked to the error that I am receiving when using the sub.sample tool?
I also tried contacting a Galaxy administrator on Friday, October 11, 2019 for assistance at firstname.lastname@example.org, however I have not received a response. I was unable to locate contact information for a Galaxy administrator or for technical support.
I was wondering if there are additional recommendations on addressing this challenge or for contacting an Galaxy administrator for help.
Thank you for your time and I look forward to hearing from you.