Data paused and keep getting error: Tophat RNA data -- Tophat/Tophat2 Deprecated, use HISAT2


I have uploaded some files from ncbi and trying to do multiple analysis using tophat RNA seq. It keep going into pause and then it comes up with error. can someone help please?


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Hello @ajisa_ali

The tools Tophat and Tophat2 have been deprecated for many years. HISAT2 is the replacement.

To learn more about current Transcriptomics methods and tools, please see: