Hey,
I somehow managed to get over that error in DESeq2 but now I am getting the following as new error:
2 samples with counts over 18838 genes
using disperion fit type: parametric
estimating size factors Error in checkForExperimentalReplicates(object, modelMatrix) : The design matrix has the same number of samples and coefficients to fit, so estimation of dispersion is not possible. Treating samples as replicates was deprecated in v1.20 and no longer supported since v1.22. Calls: DESeq → DESeqParallel → checkForExperimentalReplicates
Thank you,