I’m conducting an RNA-Seq analysis on galaxy and I’m trying to use limma-voom to identify differential gene expression. When I input both files, I get the following error:
Error in data.frame(sample = basename(filenamesIn), filename = filenamesIn, :
duplicate row.names: /galaxy-repl/main/files/033/345/dataset_33345502.dat
Does anyone know why I would get this error message? My previous data is an output from kallisto and there are two columns per file I input: the transcripts ID and the est_counts. The transcript IDs are a bit odd looking and they look like : ENST00000631435.1. Perhaps this is the problem. I’m unsure which headers limma-voom accepts. I’m relatively new to galaxy.and any suggestions would be helpful.