I have been recently advising a colleague on how to mine gene-specific sequences from their metagenomic sequencing data using the Blast+ tools on the web version of Galaxy. I had them convert their fastqs to fasta and then advised them to make a custom Blast database with these sequences. While Gal…

Errors with NCBI Blast+: cannot index custom database and dc-megablast throws window size errors.

jennaj December 8, 2020, 12:04am 2

Please see this related Q&A. In short, corrections have been made and you can try again if working at UseGalaxy.org.

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