I got paired-end .fastq files from Genewiz Ampicon EZ service.
Then I try to de-multiplex the files by the barcodes (in my primers) by the Barcode Splitter tool.
However, that tool returned my .fastqsanger files instead of .fastq format.
Because I want to use CRISPResso2 (CRISPResso2) to analyze my result and CRISPRresso only recognize .fastq format. Does anybody have experience converting .fastqsanger to .fastq?
I have tried Fastq Groomer tool and Fastq-sanger to Fastq Sequence Converter (http://sequenceconversion.bugaco.com/converter/biology/sequences/fastq-sanger_to_fastq.php)
But they dont seem to help, the resulting files still cannot be recognized by CRISPResso.