How to merge multiple fastq files into one? How do trim low-quality sequences based on Qscore of Nanopore data?

Dear all,

I am a new user who is working on Nanopore data. I have many fastq files for each individual sample, but I do not know how to merge these files. In addition, I am also looking for a tool for trimming low-quality sequences based on Qscore before mapping. Is there any tool compatible with Nanapore for this work?

I really appreciate any help you can provide.

Hi @Thuy_Nguyen
check GTN tutorials:

For merging try Concatenate.

Hope that helps.

Igor

1 Like

Hi @igor
Thank you for your prompt sharing. It is so helpful.

Thuy