downloaded the several bacteria genom fasta files and uploaded to Galaxy Version 0.1.0 and recieved the following result:
No tracrRNAs were found in this genome.
Command: perl TracrHotSpot.pl -in /mnt/SSD/galaxy/dependency_dir/TracrWeb_v4/tmp/dataset_48168.gbff -mfe_cutoff 20 -p_cutoff 0.975 -e_cutoff 20.0 -cd_cutoff 4.0 -tail_cut 204 -tt_skip 40 -around_cas9 3500
even uploaded streptococcus pyogene genom, got the sam result. i think i have not upload the correct file. I don’t which kind of file should be uploaded for tracrRNAs finder?
Hi @zhaozq
can you please provide more info? What server do you use? What tool do you use? The main Galaxy tool shed does not have tracrRNA, so I am somewhat confused by your post.
Kind regards,
Igor
Hi @zhaozq
Thank you for the reply! It seems it is a locally developed software. It is not available in the main and test tool shed. It means it is very unlikely to find a person with experience in this software on this forum. Contact the local support.
Kind regards,
Igor
