Identification of Cre-recombinase in ScRNAseq data

Dear All,

I would like to know, how to identify Cre-recombinase is expressed or not in my scRNAseq data? I have fastq files, Please help to guide in this. Thanks in advance.

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Hi @Barani_Rajendran,
you can use RNA STAR for mapping the Cre-recombinase sequence against your dataset. You can examine if the Cre-recombinase mapped to the sequences in the log file generated by that tool.

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