Kraken2 error message on paired-end collection. Not analyzing all samples

Hi @bgood07

As a first pass guess, the EU server was likely just busy over the weekend.

I can see a window where many Kraken2 jobs were running all at the same time in the job statistics.

Graphics for the current version of Kraken2 for the last two days:


There isn’t anything you can do on your side to get this to process in parallel differently, but I’m wondering if the EU administrators would be interested in reviewing to see if something should be adjusted on the server re concurrent jobs accessing that same shared resource. Or, maybe there was some other small known issue impacting the work. Let’s ping them here – Hi @wm75, what do you think?


What to do

Then, the advice I would have for larger batches of work at the public servers. 1. start up the processing for an entire batch. 2. Later, if some fail, click into the collection and use the rerun icon on each failed dataset. This brings up the original tool form and you can run it again for just that single sample.

Bonus: If the failed job was run as part of a workflow, right above the Run Tool button will be two new extra options. This is useful when running longer, complicated workflows, but also shorter ones. The result is indistinguishable from runs where everything worked perfectly to start with.

  • Replace elements – this sorts the new output back into the original collection output nesting (with the already successful inputs).
  • Resume dependencies – this starts up any downstream tools that were paused because of these upstream failures (assuming the new job works!)

For you or anyone reading interested in workflows: these can contain as few as one tool (plus the “inputs” definition). These don’t have to be created from scratch and can instead be templated by extracting from existing histories. This simple tutorial has a short example if interested → Hands-on: Galaxy Basics for everyone / Galaxy Basics for everyone / Introduction to Galaxy Analyses (Galaxy Management)

Let’s start there and please let us know if you have follow up questions. The EU admins may also appreciate a shared history here but they can find the jobs other ways too – you could send in a bug report from one of the failures and put a link to this topic in the comments form to link it all back together for extra context. Hope this helps! :slight_smile: