Hi, I haven’t used Galaxy LEfSe analysis for a while, and I recently tried to use it again. However, I’m unable to proceed beyond the LDA Effect Size step. I even tested an old text file that worked perfectly a couple of years ago, but now, the same file is not working, and I keep encountering an error message. I’m not sure what I might be missing.
I’ve included a link to my text file, and I would greatly appreciate it if anyone could help me resolve this issue. Thank you!
admin redacted link
Hi @jeylee
If you have an analysis error and would like feedback, you are welcome to share a link to your history here and the community can help. How to do this is in the banner of this forum, also here. → How to get faster help with your question. You can add in screenshots if those help to explain what is going on, too, but external links to text documents won’t help to solve the actual problem, so avoid those please.
All that said, if you are having a problem at this server below, you should post your question over to the dedicated forum. Maybe search there first, since there has been prior discussion that sounds similar to your issue. The tools had some problem, and alternatives were recommended. But – I don’t know the status of that now or if those are working again.
Hope this helps and you can clarify where you are working. The server URL is at the very top of your browser window. 
Update: I just checked and this topic appears to be similar to yours. → LEfSe issues and errors - #17 by garretta - LEfSe - The bioBakery help forum