Problem with Trimmomatic

1

Hi,

I have problem running the Trimmomatioc tool. I have pared ends. First, I concatenated forward files to one and reverse into another. After this, I have run Fastqc for the quality which shows the format sanger/Illumina 1.9. After running the Trimmomatic tool I got red error msg as below. Please suggest me.

Thank you

Vijay

Arguments:

  • -mx8G

  • -jar

  • /mnt/galaxy/tools/trimmomatic/0.32/pjbriggs/trimmomatic/f8a9a5eaca8a/trimmomatic-0.32.jar

  • PE

  • -threads

  • 4

  • -phred33

  • /mnt/galaxy/files/000/dataset_84.dat

  • /mnt/galaxy/files/000/dataset_83.dat

  • /mnt/galaxy/files/000/dat

2

Let’s keep everything in one topic. So, follow-up can go in here. "This is a new dataset and not all of its data are available yet" for 4 hours with trimmomatic