Hello.I am currently doing project in exome sequencing. My data is paired sample and when i do fastqc i get 4 outputs(one for forward and another for reverse i.e., raw data and webpage).
Hi @gbbio
I already saw the page but i don’t understand what is meant by “flatten collection” as i am a student who is currently studying about galaxy server and NGS techniques . Please let me know the steps for flatten collection and also where to find that option. Please help me out.
Find it at most public Galaxy servers by either navigating to the tool panel section “Collection Operations” or by searching tool panel with the keyword “flatten”.
General process:
Run the original read collection through the tool “Flatten collection”
Run FastQC on the “flattened” read collection
Run MultiQC on the FastQC outputs
Use the original read collection for other downstream tools.
Note: The original and flattened read collections both represent the same data content. The only difference is the technical formatting.