Hi @alexbisias
Usually, yes, the p-value is what is used for “score” when calculating the FDR.
The file format for narrowPeak datasets is here: Genome Browser FAQ
You’ll notice the first few columns are similar to BED format, which has a score metric in the 5th column, but what that score represents differs between the two plus narrowPeak has two more summary attributes.
Resources
-
Diffbind
- Usage from the tool form’s help section in Galaxy:
https://bioconductor.org/packages/release/bioc/vignettes/DiffBind/inst/doc/DiffBind.pdf - Bioconductor author Q&A forum: https://support.bioconductor.org/post/search/?query=diffbind
- Usage from the tool form’s help section in Galaxy:
-
MACS2
- See the README and ISSUES to start with https://github.com/macs3-project/MACS
- Google Group author Q&A: https://groups.google.com/g/macs-announcement/
-
Tutorials
- https://training.galaxyproject.org/training-material/search?query=ChIP-seq
- https://training.galaxyproject.org/training-material/search?query=macs2
- And, the author resources have more examples
The GTN and Bioconductor groups will be hosting a training later this month. Please consider joining for direct expertise help from the original tool authors and scientists from both communities Smörgåsbord 3: A week of Free, Online, Galaxy Training supported by the Global Galaxy Training Community