SnpEff build: Create Manihot esuclenta database

[jennaj]

Hi @Emili_Ishikawa_Garci

Are you working at UseGalaxy.eu? I’m asking since the resource allocation should be enough at that server.

Would you like to share a history that contains your input reference fasta and reference annotation, and the failed run? We could review for potential problems and provide feedback.

How to share your work:

• Troubleshooting resources for errors or unexpected results
• How to get faster help with your question

You might want to first review prior help, too. Remember that technical errors in the input files can lead to “false” resource errors. The solution is to fix the data formats and rerun. But we can help with format problems here if you get stuck.

This is one prior Q&A, and more can be found here snpeff

@jennaj

We don’t have a specific tutorial for this since how the tool works in Galaxy is the same as for the command-line version. That means the author resources are the help. Technically, Galaxy creates a sort of mini-database with the output but those are not exposed to users.

Some tips:

1. Use properly formatted reference data as the source
2. Review the help on the tool form, and the link outs to the author resources
3. Be careful about how you are adding labels on the tool form. The database name should be all oneWord and not starting with a number. If you run the tool several times, use a distinct database name for each run to avoid clashes. I tend to just number them as I change my mind about the parameters: species1, species2, speciesN.

Let’s start there