STAR-FUSION: "BLAST+-blastn of the reference fasta sequence with itself"- cannot generate a last-matching gene pairs file

1

Hi all,

I am using Star-fusion. To generate a last-matching gene pairs file, I download “GRCh37_gencode_v19_CTAT_lib_Mar012021.source.tar.gz” from Index of /Trinity/CTAT_RESOURCE_LIB
I run it in the NCBI BLAST+ blastn (https://usegalaxy.org/) as:

Nucleotide query sequence(s): GRCh37_gencode_v19_CTAT_lib_Mar012021.source.tar.gz
Subject database/sequences: GRCh37_gencode_v19_CTAT_lib_Mar012021.source.tar.gz
Type of BLAST: blastn - Traditional BLASTN requiring an exact match of 11, for somewhat similar sequences
Output format: Tabular (standard 12 columns)

However, i cannot generate a last-matching gene pairs file. Does anyone know what goes wrong? Many thanks.