Tophate error occured with dataset

1

Dear Team,

I am a beginner and run Tophat to align the paired-end dataset. I found an error while executing it. Plz, guide me.

error

An error occurred with this dataset:

Beginning TopHat run (v2.1.1)

Checking for Bowtie
Bowtie version: 2.2.8.0
Checking for Bowtie index files (genome)…

2

I recommand use RNA STAR for your alignment. and about this problem please take a screen shot from the main page of bowtie So i can see what are your inputs