Unable to view bams via igb nor igv

,
1

Hello!

I am trying to view my bam from galaxy history.

When i hit “display with IGV”, igv gives me the following error:

Error loading http://172.16.2.200:8080/display_application/66ea444137ed33c5/igv_bam/local_default/cb93b73357bddfaf/data/galaxy_66ea444137ed33c5.bam: An error occurred while accessing: http://172.16.2.200:8080/display_application/66ea444137ed33c5/igv_bam/local_default/cb93b73357bddfaf/data/galaxy_66ea444137ed33c5.bam Error loading BAM file: htsjdk.samtools.util.RuntimeEOFException: Premature EOF. Expected 4 but only received 0; BinaryCodec in readmode; streamed file (filename not available)

When i hit “display with IGB”, igb says nothing and shows my bam in the “data management list” but shows no reads.

However this “http://172.16.2.200:8080/display_application/66ea444137ed33c5/igv_bam/local_default/cb93b73357bddfaf/data/galaxy_66ea444137ed33c5.bam” is downloaded and shown perfectly in both browsers when i download it manually. This file is about 12 gb long.

What i am doing wrong?

Thanks in advance.

2

When i upload this file to usegalaxy.eu, all is working fine.

3

Hi, have you fixed it? I have the same issue. Is it because your host 172.16.2.200 does not allow automatic downloading?