Hello,
I’m having issue with the annotation on the table. Apart from the p values, everything is marked in NA. I’ve read on the forum to fill manually using the tools in the advanced options but it still doesn’t work. I’m using the same file I used for my mapping (bowtie2) who is in a GFF3 format but I tried with a GTF format too. I should point out that I’m studying prokaryotic genomes, so I don’t have any exons.
Open up the Advanced Options on the tool form to customize which attributes will be used from the GTF version of your annotation. The tool is using those to attach the annotation file to your data file, and if it is looking for something that is not present (like “exon”) then it might just happily output nonsense, and not necessarily fail.
Please give that a try, and if you need more help, you can share back what you have so far with your tests and we can try to help more. Sharing your history would be best for this so we can see all of the data, and offer actionable advice. How to share is in the banner at this forum, and you can post the history share link back here with your comments.
Thank you for the quick answer !
I tried that already, but unfortunately, i can’t make it work. I’m adding screenshots of the advanced settings I’ve completed
We can’t see the error message you are getting here, nor the other files involved. You are still welcome to share back your work and we can try to help with that context. Thanks!
Hello,
I don’t really have any error messages, just NAs instead of gene names and functions. I’m not comfortable making my history available to everyone. Can you send me your e-mail?