BlobToolKit problem

usegalaxy.org.au support
1

Hi!

I met a problem when using the BlobToolKit. Here is my history: Galaxy | Australia.
Here is the error message:
Traceback (most recent call last):
File “/opt/conda/envs/btk_env/bin/blobtools”, line 8, in
sys.exit(cli())
File “/opt/conda/envs/btk_env/lib/python3.9/site-packages/blobtools/blobtools.py”, line 105, in cli
sys.exit(subcommand())
File “/opt/conda/envs/btk_env/lib/python3.9/site-packages/blobtools/lib/add.py”, line 203, in cli
main(args)
File “/opt/conda/envs/btk_env/lib/python3.9/site-packages/blobtools/lib/add.py”, line 149, in main
parsed = field[“module”].parse(
File “/opt/conda/envs/btk_env/lib/python3.9/site-packages/blobtools/lib/bed.py”, line 366, in parse
filename, all_windows, full = parse_tsvfiles(files)
File “/opt/conda/envs/btk_env/lib/python3.9/site-packages/blobtools/lib/bed.py”, line 310, in parse_tsvfiles
[fullfile, *files] = sorted(files, key=len)
ValueError: not enough values to unpack (expected at least 1, got 0)

Could you help me find where the problem came from? Thank you very much!

Cheers,
Chuan

2

Hi @chuan_zhai
I don’t have much experience with BlobToolKit, and coverage file is not available in test data, so I cannot run a test, but it seems the error was caused by the coverage file. The description for coverage input says:
BED file of coverages per scaffold
It seems your coverage file has more contigs that the fasta file. Are you sure the files are for the same assembly?
Kind regards,
Igor

1 Like
3

Thanks igor! You are right. The coverge file is wrong. Thanks!