CREx_rearrangement scenario

I want to know how to use Galaxy to generate a “rearrangement scenario”?

Like Figure 1 in the paper “doi:10.1093/bioinformatics/btm468” when CREx was released, or in the instructions in the old version of the CREx interactive webpage “https://siks.informatik.uni-leipzig.de/software/crex/crex/doc/tutorial.html”.

Currently, when I use Galaxy, after inputting a fasta file containing two gene sequences, I can only get a tabular file. It seems to be a comparison file of the gene sequences of the two mitochondrial genomes, but there is no TDRL-based rearrangement process.

Welcome @yaoyuan

This is an example input/ouput https://usegalaxy.eu/u/jenj/h/test-crex. The test case didn’t include any TDRL cases, and I can’t get those I made up extra (outside the tool tests) to produce it without an error, using either version of the tool.

Let’s ask the author – Hi @bernt-matthias would you be able to help?

Dear @yaoyuan,

unfortunatellt the command line version in CREx (which is what the Galaxy tool executes) does not have this functionality. The drawing of the figures was a feature that was implemented in the old CREx webpage which unfortunately was shut down.

So, currently there is only the (equivalent) textual representation of the rearrangement. I’m happy to help if you need any help with interpreting these.

You can also share your history and I can have a look at the results of the specific use case.

Best,
Matthias