Demultiplexing option(s) for Porechop (Galaxy Version 0.2.4+galaxy1)

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Hello there eveyone!As a beginner, I was wanting to use the Porechop tool for adapter trimming and demultiplexing my fastq files.

I noticed that there is no option for selecting “Demultiplexing” parameters on the Porechop for my Galaxy tool-shed.

Can someone please assist me in understanding how I can demultiplex Long-read Nanopore data whilst also trimming the adapter sequences too?

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Welcome @d_mehta

We have a really thorough set of Single Cell analysis resources at → Single Cell / Tutorial List

We also have an upcoming training event that everyone is welcome to join. → Galaxy Training Academy 2025

Then for your specific quesion:

Hopefully you found this already! Use the Barcode binning settings option on form. The screenshot is from the tool form as displayed at the UseGalaxy.eu server for this version of the tool → Porechop adapter trimmer for Oxford Nanopore reads(Galaxy Version 0.2.4+galaxy1) (link at EU, then the same tool at UseGalaxy.org).

porechop-binning
porechop-binning862×850 60.1 KB

Details of how it works are at the developer site. This is mostly the same as in Galaxy, except you don’t need to specify the output directory.

Hope this helps! :slight_smile:

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Thank you very much @jennaj

I’m glad that I received a reply and I will check the ‘Barcode binning’ settings out.

I have started learning bioinformatics just recently so I am yet learning to have the “knack” for it. Although the options did look somewhat similar on GitHub, I was not sure if they were related or not.
I am glad to have my doubts cleared.

Thanks once again, for providing the links to the tutorials and the Training Academy along with the answer to my query!

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