Trying to use DESEQ2 for a couple of days now, I have the output of FeatureCounts, I also used Limma Voom successfully. However I need the tables with the p-value and the fold difference in expression so I try to get them via DESEQ2. I tried to compare the Control with each mutant (4) but I think I got it wrong. I’m not sure how to import the files correctly and I couldn’t find any proper explanation of the factors. Please help or an idea where to start from?