Does FeatureCount Built-in include non coding region?

,
1

I am trying to identify and study the differentially expressed non-coding RNA, I used the FeatureCount Built-in genome for the counting, but I am not getting non-coding RNA. Any help is welcome. Thanks!

1 Like
2

Hello @exotic1213

The built-in annotation sources are for coding regions of the genome.

If you want to look for ncRNA, Gencode is one annotation source. Use the GTF version of the annotation, format it correctly, and adjust the tool form setting to match the annotation features/attributes.

Annotation:
https://www.gencodegenes.org/human/

FAQs:
https://galaxyproject.org/learn/datatypes/#gtf
https://galaxyproject.org/support/diff-expression/

Tutorials:

I also added some tags to your post that point to related prior Q&A.

Hope that helps!

3

Thanks, this is very helpful!