EGSEA help - Error in plotHeatMapsLogFC

Hello guys,

I am running EGSEA, and it was working fine until I started to run into errors.

I have the file in tabular format.

Geneid FFSSCLCR3 FFSSCLCR2 FFSSCLCR1 iPSC1 iPSC2 iPSC3
2018 2867.300115 4384.063805 3697.321171 0 0 0
196047 2139.832269 3254.124667 2938.558938 0 0 3.544768899
27063 6174.085968 2953.349501 6630.032231 0 7.378716416 5.317153348

And have a factor as tabular format

Sample Cell Type
FFSSCLCR3 FFSSCLC
FFSSCLCR2 FFSSCLC
FFSSCLCR1 FFSSCLC
iPSC1 iPSC
iPSC2 iPSC
iPSC3 iPSC

and my annotation as tabular as well

ENTREZID SYMBOL
100287102 DDX11L1
653635 WASH7P
102466751 MIR6859-1
100302278 MIR1302-2
645520 FAM138A
79501 OR4F5
729737 LOC729737
102725121 DDX11L17

But… when I tried to run EGSEA, it says
groupGOTerms: GOBPTerm, GOMFTerm, GOCCTerm environments built.
EGSEA analysis has started
Log fold changes are estimated using limma package …
limma DE analysis is carried out …
EGSEA is running on the provided data and h collection
…globaltestcamera.ora*
EGSEA is running on the provided data and c5 collection
…cameraglobaltestora*
EGSEA is running on the provided data and gsdbgo collection
…globaltestcamera.ora*
EGSEA is running on the provided data and kegg collection
…globaltestcamera.ora*
EGSEA analysis took 12.34 seconds.
EGSEA analysis has completed
EGSEA HTML report is being generated …
Report pages and figures are being generated for the h collection …
Heat maps are being generated for top-ranked gene sets
based on logFC …
Error in plotHeatMapsLogFC(gene.sets = gsets.top, fc = logFC, limma.tops = limma.tops, :
All featureIDs in the gs.annot list should map to
a valid gene symbol
Calls: egsea.cnt → egsea

and tried to troubleshoot, but I gave up after three days of trying…
can anyone help me to figure out what I am doing wrong here?

Thank you in advance

Hello guys,

Could it be because I am using hg38?
Should I try with hg19 and run EGSEA again?

I tried to remove all “NA,” “LOC###,” and ‘XXX-AS’ genes from my list. It still doesn’t run, and I am running out of idea how to make it run.

Thank you,