for me, after feature counts, dseq2 is not working
Welcome, @Vijina_Chakkyarath
There is much prior troubleshooting for errors with these tools at this forum.
You can search with keywords like tool names and datatypes, or click on the tags I added.
You’ll find links to tutorials with examples, plus common solutions that have worked for others.
Please give that a review, compare your data to the data others have shared, and follow up here if you still have a specific question. We’ll need more information to help, and you’ll see that in the other topics, or can review here: Troubleshooting errors
Let’s start there. Learning how to ask questions with context will be a useful skill, too 