I am trying to convert a 5 column bed file to a four column bedgraph file that I then can convert to BigWig.
Here are the original file columns
chr1 3389605 3390648 MACS_peak_1 122.27
I used CUT to remove column 4, MACS_peak_1, and have added text to the revised file designating it as a begraph file.
Here is an example of my edit:
track type=bedGraph name=“BedGraph Format” description=“BedGraph format”
chr1 3389258 3390702 121.63
I must be doing something wrong as Galaxy’s BedGraph to BigWig conversion tool does not allow me to select this file for conversion. Can someone please make a suggestion.